The present study, discuss the role of antioxidant enzyme glutathione peroxidase to overcome free radicals produced under the influence of various stressors. A 24 hours exposure of silkworm, Bombyx mori to cold, hypoxia and nuclear polyhedral virus resulted in a significant increase in Glutathione peroxidase in the various tissue of both IV and V instars. However, upon recovery from stress, antioxidant enzyme activity returned to base value and larvae did not show any age dependent difference in glutathione peroxidase activity. All the results are discussed in the light of immediate response of poikilotherms to various stressors.
Ananda Kumar M. D.*
KSSRDI, Thalaghattapura, Bengaluru-560109, Karnataka, INDIA
Ann Sandhya Michael
Department of Life Science, Bangalore University, Bangalore- 560 056, Karnataka, INDIA
Kumar, M.D.A., Michael, A.S., 2019. Stressors induced glutathione peroxidase activity in lepidopteran insect silkworm (Bombyx mori). Innovative Farming, 4(4): 210-213.
Aebi, H. 1984. Catalase in vitro." Methods in enzymology. Academic Press, 105: 121-126.
Ahmad, S., M. A. Beilsen and S. R. Pardini. 1989. Glutathioneperoxidase activity in insects: a reassessment. Arch. Insect Biochem. Physiol, 12: 31-49.
Ahmad, S., D. L. Duval, L. C. Weinhold and S. R. Pardini. 1991. Cabbage looper antioxidant enzymes: tissue specificity. Insect Bochem, 21: 563-572.
Ahmad, S. and S. R. Pardini. 1990. Mechanism for regulating oxygen toxicity in phytophagous insects. Free Radic. Biol. Med, 8:401-413.
Ahmad, S., C. A. Pritsos and S. R. Pardini. 1990. Antioxidant enzyme activities in subcellular fractions of larvae of the black swallowtail butterfly, Papiliopolyxenes. Arch. Insect Biochem. Physiol, 15: 101-109.
Buyukguzel, E. and Y. Kalender. 2007. Pencillin induced oxidative stress: Effects on antioxidant response of midgut tissue in instars of Galleria mellonella. J. Econ. Entomol, 100:1533-1541.
Davies, K. J. 1995. Oxidative stress: the paradox of aerobic life. Biochem. Soc. Symp,
61:1-31.
Droge, W. 2002. Free radicals in the physiological control of cell function. Physiol. Rev, 82: 47-95.
Felton, G. W. and C. B. Summers. 1995. Antioxidant systems in insects. Arch. Insect Biochem. Physiol, 29: 187-197.
Flohe, L. and W. A. Gunzler. 1984. Assays of glutathione peroxidase. Methods Enzmol, 105: 115-121.
Imlay, J. A. 2008. Cellular Defenses against Superoxide and Hydrogen Peroxide. Annu. Rev. Biochem, 77: 755–76.
Joanisse, R. D. and K. B. Storey. 1996. Oxidative stress and antioxidants in overwintering larvae of cold-hardy goldenrod gall insects. J. Exp. Biol, 199: 1483–1491.
Jovanovic-Galvovic, A., D. P. Blagojevic, G. Grubor-Lajsic, M. R. Worland and M. B. Spasic. 2007. Antioxidant defense in mitochondria during diapause and post diapause development of European corn borer (Ostrinia nubilalis Hubu). Arch. Insect Biochem. Physiol, 64: 111-119.
Lee, S. R., S. R. Kim, N. S. Park, I. Kim, P. D. Kang, B. H. Solm, K. H. Choi, S. W. Kang, Y.H. Je, S. M. Lee, H. D. Solm and B. R. Jin. 2005. Characterisation of silkworm thioredoxin peroxidase that is induced by external temperature stimulus and viral infection. Insect. Biochem. Molecular Biol, 35: 73-84.
Li, B., Y. Xie, Z. Cheng, J. Cheng, R. Hu, Y. Cui, X. Gong, W. Shen and F. Hong. 2011. Effects of Added CeCl3 on Resistance of Fifth-Instar Larvae of Silkworm to Bombyx mori Nucleopolyhedrovirus Infection. Biol. Trace. Elem. Res, 146: 318-324.
Misra, H. P. and I. Fridovich. 1972. The role of superoxide anion in the autoxidation of epinephrine and a simple assay of superoxide dismutase. J. Biol. Chem, 247: 3170- 3175.
Sim, C. and D. L. Denlinger. 2011. Catalase and superoxide dismutase-2 enhance survival and protect ovaries during overwintering diapause in the mosquito Culex pipiens. J. Insect Physiol, 57: 628–634.
Sohal, R. S., Mockett, R. J., Orr, W. C., 2002. Mechanisms of aging: an appraisal of the oxidative stress hypothesis. Free Radic. Biol. Med. 33(5): 575–586.
Vertuani, S., A. Angusti and S. Manfredini. 2004. The antioxidants and pro-oxidants network: an overview. Curr. Pharm. Des, 10: 1677-1694.
Vyjayanthi, N. and M. Subramanyam. 2002. Effect of Fenvalerate – 20EC on sericigenous insect: I. Food utilization in the late age larva of silkworm B.mori. Ecotoxicol. Environ. Safety, 53: 206.
Vyjayanthi, N. and M. Subramanyam. 2002. Effect of Fenvalerate – 20EC on sericigenous insect: II. Digestive enzymes in the nutritive physiology of silkworm B.mori. Ecotoxicol. Environ. Safety, 53: 212.
Wang, Y., L. W. Oberley and D. W. Murhammer. 2001. Evidence of oxidative stress following the viral infection of two lepidopteran insect cell lines. Free Radic. Biol. Med, 31(11):1448–1445.