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<p style="text-align:justify"><span style="font-size:16px">Genetic testing is widely used method for disease diagnosis in human, animal and plant systems. Currently, PCR is a method of choice for gene amplification but requires a thermal cycler for the reaction and electrophoresis by fluorescent intercalator to detect the amplification. However, this method remains elusive only to affordable laboratories across world. Loop-mediated isothermal amplification (LAMP) is an outstanding gene amplification procedure that combines rapidity, simplicity, and high specificity. Samples are amplified at a fixed temperature through a repetition of two types of elongation reactions occurring at the loop regions. LAMP technique has wide applications, including in point-of-care testing, genetic testing in resource constrained places, and rapid testing of food products and environmental samples.</span></p>
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<p style="text-align:justify"><span style="font-size:16px"><strong>Rajesh, S.<sup>*</sup></strong></span></p> <p style="text-align:justify"><span style="font-size:16px"><em>Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu (641 003), India</em></span></p> <p style="text-align:justify"><span style="font-size:16px"><strong>Varanavasiappan, S.</strong></span></p> <p style="text-align:justify"><span style="font-size:16px"><em>Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu (641 003), India</em></span></p> <p style="text-align:justify"><span style="font-size:16px"><strong>Dhivya, K.</strong></span></p> <p style="text-align:justify"><span style="font-size:16px"><em>Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu (641 003), India</em></span></p>
How to cite
<p style="text-align:justify"><span style="font-size:14px">Rajesh, S., Varanavasiappan, S., Dhivya, K., 2020. Loop mediated isothermal amplification (LAMP) - A robust molecular tool for rapid disease diagnosis. <em> Biotica Research Today </em>2(11), 1117-1121.</span></p>
Reference
<p style="text-align:justify"><span style="font-size:16px">Notomi, T., Okayama, H., Masubuchi, H., Yonekawa, T., Watanabe, K., Amino, N., and Hase, T., 2000.Loop-mediated isothermal amplification of DNA. <em>Nucleic Acids Res.</em> 28, E63.</span></p> <p style="text-align:justify"><span style="font-size:16px">Tomita, N., Mori, Y., Kanda, H., and Notomi, T.,2008.Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products.<em>Nat. Protoc. </em>3, 877–882.</span></p> <p style="text-align:justify"><span style="font-size:16px">http://www.premierbiosoft.com/tech_notes/Loop-Mediated-Isothermal-Amplification.html</span></p>
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