By application of various conventional, mutational and molecular breeding approaches considerable improvement and high productivity of Rice (Oryza sativa L.) have been gained. However, these methods are not enough to enhance the yield as well as combating the resurgence of various biotic and extending abiotic stress conditions. So, now-a-days to boost up these factors, advanced genome editing tool like CRISPR/cas9 is on high demand. This system uses a complex of RNA-Protein (sgRNA-Cas9) to proceed with a double-strand break in the target DNA, a repair mechanism (NHEJ or HDR) and direct knocking out of the target gene. Even it can replace a desirable host gene in that position to enhance the desirable trait.
Kajal Samantara
Department of Genetics and Plant Breeding, M.S. Swaminathan School of Agriculture, Centurion University of Technology and Management, Odisha (761211), India
Sourav Ranjan Mohapatra
Division of Genetics and Tree Improvement, Forest Research Institute, Dehradun, Uttarakhand (248006), India
Udit Nandan Mishra*
Department of Bochemistry and Crop Physiology, M.S. Swaminathan School of Agriculture, Centurion University of Technology and Management, Odisha (761211), India
Chandrasekhar Sahu
Samantara, K., Mohapatra, S.R., Mishra, U.N., Sahu, C., 2020. Rice Genome Editing through CRISPR/Cas9: Where are we? Biotica Research Today 2(5 Spl), 308-309.
Huang, X. Z., Zeng, X. F., Li, J. R., and Zhao, D. G. (2017). Construction and analysis of tify1a and tify1b mutants in rice (Oryza sativa) based on CRISPR/Cas9 technology. J. Agric. Biotechnol. 25: 1003-1012.
Sun, Y., Jiao, G., Liu, Z., Zhang, X., Li, J., Guo, X., et al. (2017). Generation of high-amylose rice through CRISPR/Cas9-mediated targeted mutagenesis of starch branching enzymes. Front. Plant Sci. 8: 298.
Zhang, H., Zhang, J., Lang, Z., Ramon, J. B., and Zhu, J. K. (2017).Genome editing principles and applications for functional genomics research and crop improvement. Crit. Rev. Plant Sci. 36: 291-309.