Metabarcoding is a DNA barcoding process that allows for the simultaneous identification of several taxa within the same sample. This technique can be synonymously applied to environmental DNA (eDNA) barcoding. Primary difference of this technique and the barcoding is that metabarcoding does not focus on one specific organism whereas it aims to determine species composition within a sample. Unlike DNA barcoding, metabarcoding makes use of Next Generation Sequencing approaches for rapid assessment of species diversity.
Rajesh, S.*
Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu (641 003), India
Sudha, M.
Varanavasiappan, M.
Jayabalakrishnan, R. M.
Directorate of Natural Research Management, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu (641 003), India
Rajesh, S., Sudha, M., Varanavasiappan, M., Jayabalakrishnan, R.M., 2021. Metabarcoding: A Molecular Phylogenetic Tool for Large scale, Rapid Assessment of Species Diversity. Biotica Research Today 3(7), 570-572.
Brandt, M.I., Trouche, B., Quintric, L., Wincker, P., Poulain, J., Arnaud-Haond, S., 2020. A flexible pipeline combining clustering and correction tools for prokaryotic and eukaryotic metabarcoding. bioRxiv 717355, ver 3.0
Valentini, A., Pompanon, A.F., Taberlet, P., 2009. DNA barcoding for ecologists. Trends Ecol. Evol. 24(2), 110-117.