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<p style="text-align:justify"><span style="font-size:16px">The term DNA sequencing encompasses biochemical methods for determining the order of nucleotide bases. Sequencing of nucleic acid provides information about all the genetic information stored in the genome in the form of four nitrogen containing bases- adenine (A), guanine (G), thymine (T) and cytosine (C). For sequencing of nucleic acids various methods are used; (1) The classical methods including Sanger’s chain termination method and Maxam-Gilbert chemical degradation method, (2) Automated sequencers based on Sanger method, (3) Next generation sequencing methods which includes 454 Pyrosequencing, Ion Torrent sequencing, SOLiD sequencing and Illumina sequencing method <em>i.e.,</em> most widely used nowadays. The major application of gene sequencing lies in forensic, medical and molecular biology studies but in agriculture also plant genome mapping and sequencing has accelerated the crop improvement program.</span></p>
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<p style="text-align:justify"><span style="font-size:16px"><strong>Juhi Pandey*</strong></span></p> <p style="text-align:justify"><span style="font-size:16px"><span style="color:black"><em>Dept. of Genetics and Plant Breeding, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya, Faizabad, Uttar Pradesh (224 229), India</em></span></span></p> <p style="text-align:justify"><span style="font-size:16px"><strong>Ashim Debnath</strong></span></p> <p style="text-align:justify"><span style="font-size:16px"><span style="color:black"><em>Dept. of Genetics and Plant Breeding, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya, Faizabad, Uttar Pradesh (224 229), India</em></span></span></p> <p style="text-align:justify"><span style="font-size:16px"><strong>R. M. Tripathi</strong></span></p> <p style="text-align:justify"><span style="font-size:16px"><span style="color:black"><em>Dept. of Genetics and Plant Breeding, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya, Faizabad, Uttar Pradesh (224 229), India</em></span></span></p> <p style="text-align:justify"><span style="font-size:16px"><strong>Om Prakash Verma </strong></span></p> <p style="text-align:justify"><span style="font-size:16px"><span style="color:black"><em>Dept. of Genetics and Plant Breeding, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya, Faizabad, Uttar Pradesh (224 229), India</em></span></span></p> <p style="text-align:justify"><span style="font-size:16px"><strong>Niharika Yaduvanshi</strong></span></p> <p style="text-align:justify"><span style="font-size:16px"><span style="color:black"><em>Dept. of Genetics and Plant Breeding, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya, Faizabad, Uttar Pradesh (224 229), India</em></span></span></p>
How to cite
<p style="text-align:justify"><span style="font-size:14px">Pandey, J., Debnath, A., Tripathi, R.M., Verma, O.P., Yaduvanshi, N., 2021. DNA Sequencing and Its Types. <em>Biotica Research Today</em> 3(2), 119-121.</span></p>
Reference
<p style="text-align:justify"><span style="font-size:16px">Abed A., Légaré G., Pomerleau S., St-Cyr J., Boyle B., Belzile F.J., 2019. Genotyping-by-Sequencing on the Ion Torrent Platform in Barley. In: Harwood W. (eds) Barley. Methods in Molecular Biology, vol 1900. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8944-7_15.</span></p> <p style="text-align:justify"><span style="font-size:16px">Gonda, I., Faigenboim, A., Adler, C., Milavski, R., Karp, M., Shachter, A., Ronen, G., Baruch, K., Chaimovitsh, D., Dudai., N., 2020. The genome sequence of tetraploid sweet basil, <em>Ocimumbasilicum</em> L., provides tools for advanced genome editing and molecular breeding.<em> DNA Research</em>, Vol 27 Issue 5. October, dssa027.</span></p> <p style="text-align:justify"><span style="font-size:16px">Yang, X.X, Zhang, L, Huang, X.Q, Wu, W.X, Zhou, XQ, Li, H.Z., Liu, Y., 2019. Difference of the microbial community structure in the rhizosphere of soybean and oilseed rape based on high-throughput pyrosequencing analysis. <em>Chinese</em> 30(7), 2345-2351.</span></p>
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